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Literature summary extracted from

  • Okazaki, S.; Nakano, S.; Matsui, D.; Akaji, S.; Inagaki, K
    Asano, Y.: X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-lysine {varepsilon}-oxidase from Marinomonas mediterranea (2013), J. Biochem., 154, 233-236.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.20 sitting drop vapor diffusion method, crystal structure resolved to 1.98 A resolution, Protein Data Bank codes 3WEU and 3WEV Marinomonas mediterranea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.20 cytoplasm
-
 Marinomonas mediterranea 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.20 80880
-
 calculated from amino acid sequence Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.20 Marinomonas mediterranea F2JXJ3
-
-
1.4.3.20 Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.20 gel filtration on Sephacryl S-300, subsequent column chromatograpyh on DEAE-Toyopearl Marinomonas mediterranea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.20 dimer 2 * 80880, dimeric in solution Marinomonas mediterranea
1.4.3.20 tetramer 4 * 80880, tetrameric in crystal state Marinomonas mediterranea

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.20 cysteine tryptophylquinone
-
 Marinomonas mediterranea